Firstly, the success of a western blot depends on the user’s level of experience. From the initial sample preparation to final image analysis, each step requires different instruments, consumables, and reagents that often come from different suppliers. “It’s one of those underrated techniques because it's perhaps not the most exciting thing around to talk about, but it's one of those techniques without which we wouldn't have the answers that we have today,” says Sowmya.ĭespite its simplicity, gel electrophoresis and western blotting is a somewhat fragmented process. Unchanged for the past four decades, western blotting is a very simple, high-impact tool that helps answer some very basic but important research questions: Are there proteins present here? What proteins are present? How much of my target protein is present? As a result, western blotting has had a lasting consequence on workflows across research fields. Western blotting: A ubiquitous tool for biomolecule research She also explains how Cytiva’s Amersham™ ImageQuant™ 800 western blot imaging systems have a full suite of features aimed at supporting regulatory compliance. In this interview, Sowmya shares insight into the advancement of this fundamental technique for GxP-certified labs. “Good traceable data not only increases confidence in your final product but also helps you track and identify any issues should they ever arise.” “GxP is so very important today, especially for our customers in pharma and biopharma,” explains Sowmya Balachandran, Global Product Manager at Cytiva. Having a GxP framework in place is becoming increasingly important – and data traceability, accountability, and integrity are at the heart of this. GxP – or good practice – guidelines define and track each stage of development and manufacturing to ensure that your final product not only performs its intended purpose, but that it’s also safe for consumer use. If you’re developing a new drug, cosmetic, or food product, you’re probably familiar with GxP. Sowmya Balachandran, Global Product Manager at Cytiva, works to develop new imaging solutions & support products for use in biomolecular research LC-MS innovation: Improve analytical accuracy Which microbioreactor modules are best for you Which solid support is right for your oligo synthesis?
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